Influence of surfactants on the functioning of Micrococcus luteus 1-i strain in biofuel cells

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Abstract

The operation of biofuel cells (BFC) based on the Micrococcus luteus 1-i strain under the action of the main representatives of various groups of surfactants has been analyzed. The following were tested: cetyltrimethylammonium bromide (cationic surfactant), Tween-80 (non-ionic surfactant), sodium lauryl sulfate (anionic surfactant). It was shown that cetyltrimethylammonium bromide reduced the electrical characteristics of BFC at concentrations of 10 mg/l, Tween-80 – from 5 ml/l, sodium lauryl sulfate – from 100 mg/l. A comparison of the electrogenic activity of bacteria in BFCs with their viability and the kinetics of the redox potential of the anolyte allowed us to conclude that the decrease in the efficiency of the studied BFCs under the influence of surfactants in the tested concentration ranges is associated with their toxic effect on microbial cells.

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About the authors

D. I. Stom

Surgut State University; Baikal Museum of the SB RAS; Irkutsk National Research Technical University

Author for correspondence.
Email: stomd@mail.ru
ORCID iD: 0000-0001-9496-2961
Russian Federation, Surgut; Listvyanka, Irkutsk region; Irkutsk

I. A. Topchiy

Irkutsk State University

Email: stomd@mail.ru
ORCID iD: 0000-0002-9091-4062
Russian Federation, Irkutsk

G. O. Zhdanova

Irkutsk State University

Email: stomd@mail.ru
ORCID iD: 0000-0002-8355-9517
Russian Federation, Irkutsk

P. S. Stashkevich

Irkutsk State University

Email: stomd@mail.ru
Russian Federation, Irkutsk

K. A. Khramtsova

Irkutsk State University

Email: stomd@mail.ru
Russian Federation, Irkutsk

Yu. Yu. Petrova

Surgut State University

Email: stomd@mail.ru
ORCID iD: 0000-0003-3702-2249
Russian Federation, Surgut

R. V. Lepikash

Tula State University

Email: stomd@mail.ru
ORCID iD: 0000-0001-7853-2937
Russian Federation, Tula

A. B. Kupchinsky

Baikal Museum of the SB RAS

Email: stomd@mail.ru
ORCID iD: 0000-0001-8884-8636
Russian Federation, Listvyanka, Irkutsk region

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Supplementary files

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2. Fig. 1. The two-chamber BFC used in the work [9]: 1 – anode chamber; 2 – cathode chamber; 3 – cathode electrode; 4 – anode electrode; 5 – rubber caps that close and fix the electrodes; 6 – rubber plug in the anode chamber, through which samples are taken and substrates and bioagents are introduced using a syringe; 7 – MF-4SK proton exchange membrane.

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3. Fig. in table 1

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4. Fig. in table 2

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5. Fig. in table 3

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6. Fig. 2. Effect of cationic surfactant cetyltrimethylammonium bromide on the dynamics of voltage (measured in open circuit mode) (a) and current (b) (measured in short circuit mode) generated in a biofuel cell by the M. luteus 1-i strain (anolyte – model wastewater (substrate – peptone 500 mg/l), electrodes – carbon fabric); ● – control (without surfactant); × – CTAB 10 mg/l; ▲ – CTAB 50 mg/l; ■ – CTAB 100 mg/l; ♦ – CTAB 500 mg/l.

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7. Fig. 3. The effect of the cationic surfactant cetyltrimethylammonium bromide on the power of the studied biofuel cells during their operation under an external load (Ω) from 10 Ohm to 100 kOhm (anodic biocatalyst – strain M. luteus 1-i; anolyte – model wastewater (substrate – peptone 500 mg/l), electrodes – carbon fabric).

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8. Fig. 4. Effect of nonionic surfactant Tween-80 on the dynamics of voltage (measured in open circuit mode) (a) and current (b) (measured in short circuit mode) generated in a biofuel cell by the M. luteus 1-i strain (anolyte – model wastewater (substrate – peptone 500 mg/l), electrodes – carbon fabric); ● – control (without surfactant); × – Tween-80 5 ml/l; ▲ – Tween-80 10 ml/l; ■ – Tween-80 30 ml/l; ♦ – Tween-80 50 ml/l.

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9. Fig. 5. The effect of non-ionic surfactant tween-80 on the power of the studied biofuel cells during their operation under an external load (Ω) from 10 Ohm to 100 kOhm (anodic biocatalyst – strain M. luteus 1-i; anolyte – model wastewater (substrate – peptone 500 mg/l), electrodes – carbon fabric).

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10. Fig. 6. Effect of the anionic surfactant sodium lauryl sulfate on the dynamics of voltage (measured in open circuit mode) (a) and current (b) (measured in short circuit mode) generated in a biofuel cell by the M. luteus 1-i strain (anolyte – model wastewater (substrate – peptone 500 mg/l), electrodes – carbon fabric); ● – control (without surfactant); × – SLS 50 mg/l; ▲ – SLS 100 mg/l; ■ – SLS 500 mg/l; ♦ – SLS 1000 mg/l.

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11. Fig. 7. The effect of different concentrations of sodium lauryl sulfate on the change in the number of viable cells of M. luteus 1-i during the utilization of peptone (0.5 g/l) in BTE.

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12. Fig. 8. The effect of different concentrations of sodium lauryl sulfate on the change in the oxidation-reduction potential of the BTE anolyte with the M. luteus 1-i culture as an anode biocatalyst.

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